GrainGenes Marker Report: Xwmc335
Locus
Xwmc335
Type
Microsatellite
Chromosome
7B
Map
Svevo/Ciccio-6A 56.4 Ta-NxW-7B 37.5 Wheat-Composite2004-7B 28 Wheat_Yr79_2018-7BL 50.4
[ Show Nearby Loci ]
Map Data
Durum wheat, Svevo x Ciccio Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat_Yr79_2018
Probe
WMC335
Possible Orthologs
Xwmc335-6A Xwmc335-7B wmc335 wmc335-7B
Probe for Locus
WMC335
Locus
Xwmc335 Xwmc335-7B wmc335 wmc335-7B Xwmc335-6A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 12g3 Donor: Weibull, Svaloff Weibull Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 11 to 30 show homology to 9f4 bases 305 to 324 (20 in longest run, maxScore = 20), zone is 1-45. (CA)5G(CA)12 88 to 113.
Type
PCR SSR
PCR primers
Forward TgcggAgTAgTTcTTccccc at 36 length 20 bases, Tm 65
Reverse AcATcTTggTgAgATgcccT at 131 length 20 bases, Tm 61
SSR size
115 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC335
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc335
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