GrainGenes Marker Report: Xwmc335-7B
Locus
Xwmc335-7B
Type
Microsatellite
Chromosome
7B
Map
Ta-SSR-2004-7B 57.9 Wheat_Yr79_2018-7BL 50.4
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC335
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc335 Xwmc335-6A wmc335 wmc335-7B
Probe for Locus
WMC335
Locus
Xwmc335 Xwmc335-7B wmc335 wmc335-7B Xwmc335-6A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 12g3 Donor: Weibull, Svaloff Weibull Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 11 to 30 show homology to 9f4 bases 305 to 324 (20 in longest run, maxScore = 20), zone is 1-45. (CA)5G(CA)12 88 to 113.
Type
PCR SSR
PCR primers
Forward TgcggAgTAgTTcTTccccc at 36 length 20 bases, Tm 65
Reverse AcATcTTggTgAgATgcccT at 131 length 20 bases, Tm 61
SSR size
115 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC335
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc335-7B
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