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GrainGenes Marker Report: Xwmc336a

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Locus	Xwmc336a
Type	Microsatellite
Chromosome	1A
Map	Wheat-Composite2004-1A 22
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC336
Possible Orthologs	Xwmc336 [ Show all 7 ]
Probe for Locus	WMC336
Locus	Xwmc336a [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12a4 Donor: Weibull, Svaloff Weibull Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 112 bases located on 1D, 129 bases located on 1A Trimmed to insert at 5' end and to Rsa1 at 3' end. (GCC)7 165 to 185.
Type	PCR SSR
PCR primers	Forward gTcTTAccccgcgATcTgc at 102 length 19 bases, Tm 65 Reverse gcggccTgAgcTTcTTgAg at 196 length 19 bases, Tm 65
SSR size	113 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC336
Source Species	Triticum aestivum