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GrainGenes Marker Report: Xwmc339-1D

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Locus
Xwmc339-1D
Type
Microsatellite
Chromosome
1D
Map
Ta-SSR-2004-1D56.1
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC339
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
wmc339



Probe for Locus
WMC339
Locus
Xwmc339-1D
wmc339
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 12b5
Donor: Weibull, Svaloff Weibull
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 208 bases located on 6B
Trimmed to Rsa1 at 5' end and to insert at 3' end. (GA)8 118 to 133.
Type
PCR
SSR
PCR primers
Forward ccgcTcgccTTcTTccAg at 29 length 18 bases, Tm 66
Reverse TccggAAcATgccgATAc at 215 length 18 bases, Tm 64
SSR size
204 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence
WMC339
Source Species
Triticum aestivum