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GrainGenes Marker Report: Xwmc344-2B

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Locus	Xwmc344-2B
Type	Microsatellite
Chromosome	2B
Map	Conan/Reeder-2B 109.881 Ta-SSR-2004-2B 60.2
	[ Show Nearby Loci ]
Map Data	Wheat, Conan x Reeder Wheat, Consensus SSR, 2004
Probe	WMC344
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc344 wmc344-2B wmc344
Probe for Locus	WMC344
Locus	Xwmc344-2B Xwmc344 wmc344-2B wmc344
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12a6 Donor: Weibull, Svaloff Weibull Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)16 189 to 220.
Type	PCR SSR
PCR primers	Forward ATTTcAgTcTAATTAgcgTTgg at 80 length 22 bases, Tm 57 Reverse AAcAAAgAAcATAATTAAcccc at 304 length 22 bases, Tm 56
SSR size	246 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide (multi-locus polymorphic)
Sequence	WMC344
Source Species	Triticum aestivum