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GrainGenes Marker Report: Xwmc346

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Locus	Xwmc346
Type	Microsatellite
Map	Ta-NxW-7A 269.7
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai
Probe	WMC346
Probe for Locus	WMC346
Locus	Xwmc346
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12g6 Donor: Weibull, Svaloff Weibull Trimmed to Rsa1 at both ends. (GA)8 299 to 314, (GT)13 7 to 32,
Type	PCR SSR
PCR primers	Forward cTgAAgTTccAgccAAcAcA at 214 length 20 bases, Tm 62 Reverse ATTcccTcATcccgTTgc at 400 length 17 bases, Tm 65
SSR size	203 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence	WMC346
Source Species	Triticum aestivum