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GrainGenes Marker Report: Xwmc349

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Locus	Xwmc349
Type	Microsatellite
Chromosome	4B
Map	Ta-NxW-4B 49 Wheat, Avalon x Cadenza 4B1 34.5 Wheat-Composite2004-4B 62
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Map Data	Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMC349
Possible Orthologs	Xwmc349-4B wmc349
Probe for Locus	WMC349
Locus	Xwmc349-4B Xwmc349 wmc349
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19a2 Donor: Pioneer Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 102 bases located on 4B Trimmed to insert at both ends. (GT)22 123 to 166.
Type	PCR SSR
PCR primers	Forward AcAcAcAcTcgATcgcAc at 86 length 18 bases, Tm 59 Reverse gcAgTTgATcATcAAAAcAcA at 162 length 21 bases, Tm 60
SSR size	94 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.
Sequence	WMC349
Source Species	Triticum aestivum