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GrainGenes Marker Report: Xwmc356-2B

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Locus	Xwmc356-2B
Type	Microsatellite
Chromosome	2B
Map	Ta-SSR-2004-2B 117
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Map Data	Wheat, Consensus SSR, 2004
Probe	WMC356
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	wmc356
Probe for Locus	WMC356
Locus	Xwmc356-2B wmc356
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19c4 Donor: Pioneer Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 245 bases located on 2A, 2B Trimmed to insert at both ends. (GT)23 146 to 191, (GT)8 206 to 221.
Type	PCR SSR
PCR primers	Forward gccgTTgcccAATgTAgAAg at 67 length 20 bases, Tm 65 Reverse ccAgAgAAAcTcgccgTgTc at 289 length 20 bases, Tm 65
SSR size	242 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (there appear to be nulls of this locus in many wheats).
Sequence	WMC356
Source Species	Triticum aestivum