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GrainGenes Marker Report: Xwmc36

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Locus	Xwmc36
Type	Microsatellite
Chromosome	1D
Map	Wheat-Composite2004-1D 71
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC36
Possible Orthologs	Xwmc36-1D wmc36
Probe for Locus	WMC36
Locus	Xwmc36 Xwmc36-1D wmc36
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2a12 Donor: Wang, Utah Several microsatellites, one (CA)36(GT)36 383 to 454. Multiple inserts (2) in this fragment (joined by linker 300-313). First insert is member of 1G1, 2F8 2D11, 2H11, 2A12, 6G1, 6H3, 6H4, 6B11, 7e3, 7h5, 7b6, 8a11, 5h10, 5d12, 11d4, 9H3. Primer designed against second insert 19.6.97. Bases 33 to 87 show homology to 25a1 bases 62 to 116 (complement) (55 in longest run, maxScore = 28). Duplicate with 25a1, 2a12 has priority.
Type	PCR SSR
PCR primers	Forward TTcTcTTTTccTTTcgcAcTcc at 335 length 22 bases, Tm 64 Reverse cATcAgTTgTggggTTTcTTcA at 474 length 22 bases, Tm 64
SSR size	161 bases expected, Chinese Spring
Sequence	WMC36
Source Species	Triticum aestivum