GrainGenes Marker Report: Xwmc360-2B
Locus
Xwmc360-2B
Type
Microsatellite
Map
CS/SQ1-2B 165.4
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1
Probe
WMC360
Possible Orthologs
Xwmc360
Probe for Locus
WMC360
Locus
Xwmc360-2B Xwmc360
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 19e5 Donor: Pioneer Trimmed to insert at both ends. (GT)22 54 to 97. Software struggled to get these primers.
Type
PCR SSR
PCR primers
Forward TATgTgTgTgAAAAATgg at 2 length 18 bases, Tm 50
Reverse AATTTcTTgTAgAAccgA at 136 length 18 bases, Tm 50
SSR size
152 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product; 51C anneal, polymorphic on acrylamide.
Sequence
WMC360
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc360-2B
|
| ||||
|
| ||||
|
| ||||
| |||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
|