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GrainGenes Marker Report: Xwmc361

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Locus	Xwmc361
Type	Microsatellite
Chromosome	2B
Map	Wheat, Finch x Eltan 2B.1 7 Wheat, UC1110 x PI610750 2B 134.6 Wheat-Composite2004-2B 108
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Finch x Eltan Wheat, UC1110 x PI610750
Probe	WMC361
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc361-2B WMC361 wmc361 wmc361-2B
Probe for Locus	WMC361
Locus	Xwmc361-2B Xwmc361 WMC361 wmc361 wmc361-2B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19e6 Donor: Pioneer Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 221 bases located on 2B Trimmed to insert at both ends. (CA)10 327 to 346.
Type	PCR SSR
PCR primers	Forward AATgAAgATgcAAATcgAcggc at 214 length 22 bases, Tm 67 Reverse ATTcTcgcAcTgAAAAcAgggg at 408 length 22 bases, Tm 66
SSR size	216 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC361
Source Species	Triticum aestivum