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GrainGenes Marker Report: Xwmc363

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Locus	Xwmc363
Type	Microsatellite
Chromosome	5B
Map	Svevo/Ciccio-2B 132.9 Wheat-Composite2004-5B 30
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Breakpoint Interval	C-2BL6-0.89
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004
Probe	WMC363
Possible Orthologs	Xwmc363-2B Xwmc363-5B wmc363 wmc363-5B
Probe for Locus	WMC363
Locus	Xwmc363 Xwmc363-5B wmc363 Xwmc363-2B wmc363-5B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 19h6 Donor: Pioneer Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 120 bases located on 5B Trimmed to insert at both ends. (GT)16 40 to 71.
Type	PCR SSR
PCR primers	Forward TcTgTAAcgcATAATAgAATAgccc at 3 length 25 bases, Tm 61 Reverse ATgATTgcgTTATcTTcATATTTgg at 86 length 25 bases, Tm 62
SSR size	108 bases expected, Chinese Spring
Sequence	WMC363
Source Species	Triticum aestivum