GrainGenes Marker Report: Xwmc364-7B
Locus
Xwmc364-7B
Type
Microsatellite
Chromosome
7B
Map
CS/SQ1-7B 32.9 Ta-SSR-2004-7B 60
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004
Probe
WMC364
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc364a wmc364
Probe for Locus
WMC364
Locus
Xwmc364-7B Xwmc364a wmc364
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 12d7 Donor: Mohler, Munich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 188 bases located on 7D, 207 bases located on 7B Trimmed to Rsa1 at both ends. Bases 32 to 41 (complement) show homology to 13e1 bases 81 to 90 (10 in longest run, maxScore = 29). (CA)18 93 to 128.
Type
PCR SSR
PCR primers
Forward ATcAcAATgcTggcccTAAAAc at 41 length 22 bases, Tm 64
Reverse cAgTgccAAAATgTcgAAAgTc at 223 length 22 bases, Tm 64
SSR size
204 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide.
Sequence
WMC364
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc364-7B
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