GrainGenes Marker Report: Xwmc376-5B
Locus
Xwmc376-5B
Type
Microsatellite
Chromosome
5B
Map
Louise/Penawawa-5B 18.1 Ta-SSR-2004-5B 60
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa
Probe
WMC376
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc376 wmc376 wmc376-5B
Probe for Locus
WMC376
Locus
Xwmc376-5B Xwmc376 wmc376 wmc376-5B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 12a12.2 Donor: Mohler, Munich Multiple inserts in this clone. This is the second insert. Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 65 to 115 (complement) show homology to 11b2 bases 64 to 114 (51 in longest run, maxScore = 69). (GT)16 115 to 146, (GT)17 201 to 234.
Type
PCR SSR
PCR primers
Forward TcTcAAccAccgAcTTgTAA at 33 length 20 bases, Tm 59
Reverse AcATgTAATTggggAcAcTg at 235 length 20 bases, Tm 58
SSR size
222 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide.
Sequence
WMC376
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc376-5B
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