GrainGenes Marker Report: Xwmc386-5B
Locus
Xwmc386-5B
Type
Microsatellite
Chromosome
5B
Map
Ta-SSR-2004-5B 60.1
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC386
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
wmc386
Probe for Locus
WMC386
Locus
Xwmc386-5B wmc386
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 10f9 Donor: Jacquemin, Gembloux Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 130 bases located on 7D, 140 bases located on 7A, 176 bases located on 7B No trimming done to sequence at Agrogene, ends not located. (GT)22 102 to 145.
Type
PCR SSR
PCR primers
Forward ATcAcTgAAAcgAAATgAgcgg at 16 length 22 bases, Tm 65
Reverse TggTTggcggTTTTTcTcTAcA at 159 length 22 bases, Tm 65
SSR size
165 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence
WMC386
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc386-5B
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