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GrainGenes Marker Report: Xwmc391

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Locus	Xwmc391
Type	Microsatellite
Synonym	Other name XAWM391
Chromosome	1B
Map	Wheat-Composite2004-1B 28
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Map Data	Wheat, Composite, 2004
Probe	WMC391
Probe for Locus	WMC391
Locus	Xwmc391
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10h10 Donor: Jacquemin, Gembloux Trimmed to Rsa1 at 5' end , 3' end not located. Bases 127 to 191 (complement) show homology to 3A1 bases 668 to 732 (65 in longest run, maxScore = 124). (CT)16 24 to 55. Bases 127 to 179 show homology to 24c2 bases 124 to 176 (53 in longest run, maxScore = 102), duplicate, 10h10 has priority.
Type	PCR SSR
PCR primers	Forward gTAccAgTAgcTATgTgTTTgAT at 1 length 23 bases, Tm 55 Reverse TTAcAAgAgAgTAgAggggAAg at 124 length 23 bases, Tm 56
SSR size	145 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear.
Sequence	WMC391
Source Species	Triticum aestivum