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GrainGenes Marker Report: Xwmc395

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Locus
Xwmc395
Type
Microsatellite
Synonym
Other nameXAWM395
Chromosome
3B
Map
Wheat-Composite2004-3B46
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Map Data
Wheat, Composite, 2004
Probe
WMC395
Possible Orthologs
wmc395



Probe for Locus
WMC395
Locus
Xwmc395
wmc395
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 10f12
Donor: Jacquemin, Gembloux
Ns in sequence may be Ts. Trimmed to Rsa1 at 5' end, 3' end not located. Bases 114 to 159 show homology to 7d11 bases 96 to 141 (46 in longest run, maxScore = 67). (CT)7 87 to 100, (CT)9 112 to 129.
Type
PCR
SSR
PCR primers
Forward TccAcAcAcATAgAcgcAAAgA at 21 length 22 bases, Tm 63
Reverse AcTgccAggAcTgTTgATgAAT at 176 length 22 bases, Tm 63
SSR size
177 bases expected, Chinese Spring
Amplification Conditions
61C anneal, smear.
Sequence
WMC395
Source Species
Triticum aestivum