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GrainGenes Marker Report: Xwmc399-4D

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Locus	Xwmc399-4D
Type	Microsatellite
Chromosome	4D
Map	Ta-SSR-2004-4D 54
	[ Show Nearby Loci ]
Map Data	Wheat, Consensus SSR, 2004
Probe	WMC399
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc399 wmc399 wmc399-4D
Probe for Locus	WMC399
Locus	Xwmc399 Xwmc399-4D wmc399 wmc399-4D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22a8 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 174 bases located on 4D Trimmed to insert at both ends. (CA)13 260 to 285.
Type	PCR SSR
PCR primers	Forward cTTcAgAgATgTTTgATTAccT at 137 length 22 bases, Tm 55 Reverse ggTATTgcTAAcTgAATgATgT at 287 length 22 bases, Tm 55
SSR size	172 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product.
Sequence	WMC399
Source Species	Triticum aestivum