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GrainGenes Marker Report: Xwmc406-1B

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Locus
Xwmc406-1B
Type
Microsatellite
Chromosome
1B
Map
CS/SQ1-1B32
Kofa/UC1113-1B15.4
Ta-SSR-2004-1B23.5
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113
Durum wheat, Svevo x Ciccio
Wheat, Chinese Spring x SQ1
Wheat, Consensus SSR, 2004
Probe
WMC406
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc406
wmc406
wmc406-1B



Probe for Locus
WMC406
Locus
Xwmc406-1B
Xwmc406
wmc406
wmc406-1B
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 22b12
Donor: Romestant, RAGT
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 227 bases located on 1B
Trimmed to Ssp1 at both ends of insert. (CA)16 188 to 219.
Type
PCR
SSR
PCR primers
Forward TATgAgggTcggATcAATAcAA at 116 length 22 bases, Tm 61
Reverse cgAgTTTAcTgcAAAcAAATgg at 311 length 22 bases, Tm 61
SSR size
217 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (though weak).
Sequence
WMC406
Source Species
Triticum aestivum