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GrainGenes Marker Report: Xwmc407-2A

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Locus
Xwmc407-2A
Type
Microsatellite
Chromosome
2A
Map
T.b.xT.m.-2A127.6
Ta-SSR-2004-2A7.7
[ Show Nearby Loci ]
Map Data
T. boeoticum x monococcum
Wheat, Consensus SSR, 2004
Probe
WMC407
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc407
[ Show all 10 ]



Probe for Locus
WMC407
Locus
Xwmc407
[ Show all 11 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 10a2
Donor: Gale, John Innes
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 137 bases located on 2A
Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)16 100 to 131.
Type
PCR
SSR
PCR primers
Forward ggTAATTcTAggcTgAcATATgcTc at 30 length 25 bases, Tm 61
Reverse cATATTTccAAATccccAAcTc at 143 length 22 bases, Tm 61
SSR size
135 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (136-138 bases).
Sequence
WMC407
Source Species
Triticum aestivum