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GrainGenes Marker Report: Xwmc413-4B

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Locus	Xwmc413-4B
Type	Microsatellite
Chromosome	4B
Map	Ta-SSR-2004-4B 41.7
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Map Data	Wheat, Consensus SSR, 2004
Probe	WMC413
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc413 wmc413
Probe for Locus	WMC413
Locus	Xwmc413 Xwmc413-4B wmc413
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10g6 Donor: Gale, John Innes Trimmed to Rsa1 at both ends. Bases 13 to 39 show homology to 10g5 bases 10 to 36 (27 in longest run, maxScore = 48). (GT)9 91 to 108. NB note bias in base frequency.
Type	PCR SSR
PCR primers	Forward cAcTggAAAcATcTcTTcAAcT at 52 length 22 bases, Tm 58 Reverse AcAggAAAggATgATgTTcTcT at 192 length 22 bases, Tm 59
SSR size	162 bases expected, Chinese Spring
Amplification Conditions	tested 51C anneal, no product.
Sequence	WMC413
Source Species	Triticum aestivum