Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xwmc415b

[Submit comment/correction]

Locus	Xwmc415b
Type	Microsatellite
Chromosome	5A 5B
Chromosome Arm	5BL
Map	Tt-LxG-5B 77 Wheat-Composite2004-5A 61
	[ Show Nearby Loci ]
Breakpoint Interval	5AL12-0.35-0.78
Map Data	T. turgidum, Langdon x G18-16 Wheat, Composite, 2004
Probe	WMC415
Possible Orthologs	Xwmc415 [ Show all 18 ]
Probe for Locus	WMC415
Locus	Xwmc415a [ Show all 19 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 23e1 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 130 bases located on 5B, 142 bases located on 5A, 168 bases located on 5B Trimmed to Ssp1 at both ends of insert. (CA)23 271 to 316.
Type	PCR SSR
PCR primers	Forward AATTcgATAccTcTcAcTcAcg at 184 length 22 bases, Tm 60 Reverse TcAAcTgcTAcAAccTAgAccc at 323 length 22 bases, Tm 60
SSR size	161 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide;
Sequence	WMC415
Source Species	Triticum aestivum