GrainGenes Marker Report: Xwmc419
Locus
Xwmc419
Type
Microsatellite
Synonym
Other name XAWM419
Chromosome
4D
Map
Svevo/Ciccio-4B 44.1 Ta-NxW-1B 92.9 Wheat, Avalon x Cadenza 1B 67.1 Wheat-Composite2004-4D 42
[ Show Nearby Loci ]
Breakpoint Interval
4BS8-0.57-1.00
Map Data
Durum wheat, Svevo x Ciccio Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe
WMC419
Possible Orthologs
Xwmc419-1B
[ Show all 7 ]
Probe for Locus
WMC419
Locus
Xwmc419-1B
[ Show all 8 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 21e1 Donor: Langridge, CRC Australia Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 145 bases located on 1B No trimming done to sequence at Agrogene, ends not located. Bases 121 to 143 (complement) show homology to 1H5 bases 223 to 245 (23 in longest run, maxScore = 25). (GA)16 111 to 142.
Type
PCR SSR
PCR primers
Forward gTTTcggATAAAAccggAgTgc at 36 length 22 bases, Tm 65
Reverse AcTAcTTgTgggTTATcAccAgcc at 153 length 24 bases, Tm 65
SSR size
141 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (122-168 bases)
Sequence
WMC419
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc419
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
|