GrainGenes Marker Report: Xwmc420-2A
Locus
Xwmc420-2A
Type
Microsatellite
Map
T.b.xT.m.-2A 96
[ Show Nearby Loci ]
Map Data
T. boeoticum x monococcum
Probe
WMC420
Possible Orthologs
Xwmc420 Xwmc420-4A wmc420 wmc420-4A
Probe for Locus
WMC420
Locus
Xwmc420-4A Xwmc420 Xwmc420-2A wmc420 wmc420-4A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 21a2 Donor: Langridge, CRC Australia Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 126 bases located on 4A Trimmed to insert at 3' end, 5' end not located. (GT)22 62 to 105, (GT)4 108 to 115.
Type
PCR SSR
PCR primers
Forward ATcgTcAAcAAAATcTgAAgTg at 15 length 22 bases, Tm 59
Reverse TTAcTTTTgcTgAgAAAAcccT at 118 length 22 bases, Tm 59
SSR size
125 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (120-124 bases)
Sequence
WMC420
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc420-2A
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