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GrainGenes Marker Report: Xwmc420-4A

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Locus	Xwmc420-4A
Type	Microsatellite
Chromosome	4A
Map	Conan/Reeder-4A 18.4516 CS/SQ1-4A 164.1 Ta-SSR-2004-4A 7.1
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Map Data	Wheat, Chinese Spring x SQ1 Wheat, Conan x Reeder Wheat, Consensus SSR, 2004
Probe	WMC420
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc420 Xwmc420-2A wmc420 wmc420-4A
Probe for Locus	WMC420
Locus	Xwmc420-4A Xwmc420 Xwmc420-2A wmc420 wmc420-4A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21a2 Donor: Langridge, CRC Australia Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 126 bases located on 4A Trimmed to insert at 3' end, 5' end not located. (GT)22 62 to 105, (GT)4 108 to 115.
Type	PCR SSR
PCR primers	Forward ATcgTcAAcAAAATcTgAAgTg at 15 length 22 bases, Tm 59 Reverse TTAcTTTTgcTgAgAAAAcccT at 118 length 22 bases, Tm 59
SSR size	125 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (120-124 bases)
Sequence	WMC420
Source Species	Triticum aestivum