GrainGenes Marker Report: Xwmc421.1
Locus
Xwmc421.1
Type
Microsatellite
Map
Ta-NxW-5B 270.1
[ Show Nearby Loci ]
Map Data
Wheat, Nanda2419 x Wangshuibai
Probe
WMC421
Possible Orthologs
Xwmc42 Xwmc42-7D Xwmc421.2 Xwmc421.3
Probe for Locus
WMC421
Locus
Xwmc421.1 Xwmc421.2 Xwmc421.3
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 21a3 Donor: Langridge, CRC Australia No trimming done to sequence at Agrogene, ends not located. (GA)20 110 to 149, (GT)14 82 to 109.
Type
PCR SSR
PCR primers
Forward AAggTTAgTcgTcTcTTggAAA at 12 length 22 bases, Tm 59
Reverse TggAcTAcATgTccATgTATTg at 192 length 22 bases, Tm 58
SSR size
202 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (202 plus 210-230 bases).
Sequence
WMC421
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc421.1
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