GrainGenes Marker Report: Xwmc428-3A
Locus
Xwmc428-3A
Type
Microsatellite
Chromosome
3A
Map
Kofa/UC1113-3A 64.4 Ta-SSR-2004-3A 55.7
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Durum wheat, Svevo x Ciccio Wheat, Consensus SSR, 2004
Probe
WMC428
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc428 wmc428-3A wmc428
Probe for Locus
WMC428
Locus
Xwmc428-3A Xwmc428 wmc428-3A wmc428
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 24a3 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 259 bases located on 3A Trimmed to insert at both ends. Bases 166 to 263 (complement) show homology to 11g10.1 bases 378 to 475 (98 in longest run, maxScore = 86). (CA)25 186 to 235, (CA)4 247 to 254.
Type
PCR SSR
PCR primers
Forward TTAATccTAgccgTcccTTTTT at 39 length 22 bases, Tm 62
Reverse cgAccTTcgTTggTTATTTgTg at 274 length 22 bases, Tm 63
SSR size
257 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide.
Sequence
WMC428
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc428-3A
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