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GrainGenes Marker Report: Xwmc429

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Locus	Xwmc429
Type	Microsatellite
Chromosome	1D
Map	Ta-NxW-1D 53.4 Wheat, UC1110 x PI610750 1D 23.2 Wheat-Composite2004-1D 53
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC429
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc429-1D wmc429
Probe for Locus	WMC429
Locus	Xwmc429-1D Xwmc429 wmc429
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24c3 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 229 bases located on 1D Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 274 to 296 show homology to 11b12 bases 459 to 481 (23 in longest run, maxScore = 69). (GT)31 156 to 217, Bases 173 to 228 show homology to 21f10 bases 24 to 79 (complement) (56 in longest run, maxScore = 20). Bases 6 to 34 show homology to 25c9 bases 322 to 350 (complement) (29 in longest run, maxScore = 25).
Type	PCR SSR
PCR primers	Forward cgTAAAgATTTTcATTTggcg at 41 length 21 bases, Tm 61 Reverse AAcggcAgcTTgAAAAcATAg at 245 length 21 bases, Tm 61
SSR size	225 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC429
Source Species	Triticum aestivum