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GrainGenes Marker Report: Xwmc432

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Locus	Xwmc432
Type	Microsatellite
Chromosome	1D
Map	Wheat, Finch x Eltan 1D.2 19.8 Wheat-Composite2004-1D 13
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Map Data	Wheat, Composite, 2004 Wheat, Finch x Eltan
Probe	WMC432
Possible Orthologs	Xwmc432-1D wmc432
Probe for Locus	WMC432
Locus	Xwmc432-1D Xwmc432 wmc432
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 23a2 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 135 bases located on 4D, 191 bases located on 1D Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)14 238 to 265.
Type	PCR SSR
PCR primers	Forward ATgAcAccAgATcTAgcAc at 92 length 19 bases, Tm 53 Reverse AATATTggcATgATTAcAcA at 261 length 20 bases, Tm 54
SSR size	189 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (very complex).
Sequence	WMC432
Source Species	Triticum aestivum