GrainGenes Marker Report: Xwmc432-1D
Locus
Xwmc432-1D
Type
Microsatellite
Chromosome
1D
Map
CS/SQ1-1D 32.9 Ta-SSR-2004-1D 22.5
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004
Probe
WMC432
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc432 wmc432
Probe for Locus
WMC432
Locus
Xwmc432-1D Xwmc432 wmc432
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 23a2 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 135 bases located on 4D, 191 bases located on 1D Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)14 238 to 265.
Type
PCR SSR
PCR primers
Forward ATgAcAccAgATcTAgcAc at 92 length 19 bases, Tm 53
Reverse AATATTggcATgATTAcAcA at 261 length 20 bases, Tm 54
SSR size
189 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (very complex).
Sequence
WMC432
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc432-1D
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