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GrainGenes Marker Report: Xwmc438-7D

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Locus	Xwmc438-7D
Type	Microsatellite
Chromosome	7D
Map	Ta-SSR-2004-7D 83.8
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Map Data	Wheat, Consensus SSR, 2004
Probe	WMC438
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Probe for Locus	WMC438
Locus	Xwmc438-7D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24f5 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 261 bases located on 7D Trimmed to insert at both ends. (CA)17 106 to 139, (CA)9 142 to 159.
Type	PCR SSR
PCR primers	Forward gAccgTTgggcTgTATAgcATT at 8 length 22 bases, Tm 64 Reverse cTcTgAcAgTggTggAgcTTgA at 242 length 22 bases, Tm 65
SSR size	256 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC438
Source Species	Triticum aestivum