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GrainGenes Marker Report: Xwmc44-1B

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Locus	Xwmc44-1B
Type	Microsatellite
Chromosome	1B
Chromosome Arm	1BL
Map	P91193D1/P92201D5-1B 326.5 Ta-SSR-2004-1B 91.5 Ta-Synthetic/Opata-GPW-1B 122.8 Ta-Synthetic/Opata-SSR2-1B 119 Triticum-AxF-1B 159.2
	[ Show Nearby Loci ]
Map Data	Durum wheat, Svevo x Ciccio [ Show all 6 ]
2 Point Data	Lr46/Xwmc44-1B Distance: 15 cM
Species	Triticum aestivum
Probe	WMC44
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs	Xwmc44 wmc44 wmc44-1B
Probe for Locus	WMC44
Locus	Xwmc44-1B [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6e2 Donor: Dubcovsky, Buenos Aires Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 232 bases located on 1B AGR61. Trimmed to Rsa1 at 3' end. (GT)35 341 to 410. 5 polymorphic loci.
Type	PCR SSR
PCR primers	Forward ggTcTTcTgggcTTTgATccTg at 237 length 22 bases, Tm 66 Reverse TgTTgcTAgggAcccgTAgTgg at 457 length 22 bases, Tm 67
SSR size	242 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC44
Source Species	Triticum aestivum