GrainGenes Marker Report: Xwmc441-2B
Locus
Xwmc441-2B
Type
Microsatellite
Chromosome
2B
Map
Louise/Penawawa-2B 13.7 Ta-SSR-2004-2B 76.8
[ Show Nearby Loci ]
Map Data
Durum wheat, Svevo x Ciccio Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa
Probe
WMC441
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc441
[ Show all 7 ]
Probe for Locus
WMC441
Locus
Xwmc441-2B
[ Show all 8 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 24d6 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 159 bases located on 2B Trimmed to insert at both ends. Bases 144 to 164 show homology to 17h7 bases 131 to 151 (21 in longest run, maxScore = 38). (CA)10 53 to 72, (CG)9 73 to 90, (CT)9 35 to 52.
Type
PCR SSR
PCR primers
Forward TccAgTAgAgcAccTTTcATT at 5 length 21 bases, Tm 58
Reverse ATcAcgAAgATAAAcAAAcgg at 142 length 21 bases, Tm 58
SSR size
158 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (complex).
Sequence
WMC441
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc441-2B
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