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GrainGenes Marker Report: Xwmc446-4A

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Locus	Xwmc446-4A
Type	Microsatellite
Chromosome	4A
Map	Ta-SSR-2004-4A 9.1
	[ Show Nearby Loci ]
Map Data	Wheat, Consensus SSR, 2004
Probe	WMC446
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	wmc446 [ Show all 7 ]
Probe for Locus	WMC446
Locus	Xwmc446 [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24e7 Donor: ITCF Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)9 33 to 50, (CA)3 52 to 57. Too difficult to make primer.
Type	PCR SSR
PCR primers	Forward ccAgcTAgTAcTcTATATcTAcATc at 9 length 25 bases, Tm 52 Reverse TATTTgAAcAAgAgTTATgTgg at 103 length 21 bases, Tm 54
SSR size	115 bases expected, Chinese Spring
Amplification Conditions	51C anneal, stuttery product.
Sequence	WMC446
Source Species	Triticum aestivum