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GrainGenes Marker Report: Xwmc453-2D

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Locus	Xwmc453-2D
Type	Microsatellite
Chromosome	2D
Map	Ta-SSR-2004-2D 42.9 Wheat_BranchedSpike_2009_2D 23.1
	[ Show Nearby Loci ]
Map Data	Wheat, Consensus SSR, 2004
Probe	WMC453
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc453 [ Show all 12 ]
Probe for Locus	WMC453
Locus	Xwmc453a-2A [ Show all 13 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2c2 Donor: IGS BioTech Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172 bases located on 2A Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)35 119 to 188.
Type	PCR SSR
PCR primers	Forward AcTTgTgTccATAAccgAccTT at 73 length 22 bases, Tm 61 Reverse ATcTTTTgAggTTAcAAcccgA at 238 length 22 bases, Tm 61
SSR size	187 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (though complex).
Sequence	WMC453
Source Species	Triticum aestivum