GrainGenes Marker Report: Xwmc455-2A
Locus
Xwmc455-2A
Type
Microsatellite
Chromosome
2A
Map
Ta-SSR-2004-2A 59.3
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC455
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc455 wmc455
Probe for Locus
WMC455
Locus
Xwmc455 Xwmc455-2A wmc455
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 2b4 Donor: IGS BioTech Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 115 bases located on 2A, 168 bases located on 2A Trimmed to Rsa1 at both ends of insert. Bases 232 to 262 show homology to 12c7 bases 55 to 85 (31 in longest run, maxScore = 24). (CA)6 219 to 230, (CA)15 233 to 262, (CA)5 265 to 274.
Type
PCR SSR
PCR primers
Forward gcgTcATTTccTcAAAcAcATc at 184 length 22 bases, Tm 63
Reverse AgAAggAgAAgTgccTcAccAA at 320 length 22 bases, Tm 64
SSR size
158 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product.
Sequence
WMC455
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc455-2A
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