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GrainGenes Marker Report: Xwmc457

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Locus	Xwmc457
Type	Microsatellite
Chromosome	4D
Map	Wheat, UC1110 x PI610750 4D 63.5 Wheat, UC1110 x PI610750 4D 63.8 Wheat-Composite2004-4D 47
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Map Data	Wheat, Composite, 2004 Wheat, UC1110 x PI610750
Probe	WMC457
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc457-4D wmc457
Probe for Locus	WMC457
Locus	Xwmc457-4D Xwmc457 wmc457
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2d5.1 Donor: IGS BioTech Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 164 bases located on 4D,4A No overlap between forward and reverse sequences. Multple inserts in this clone, this is the first insert. Trimmed to insert at both ends. (CA)13 342 to 367.
Type	PCR SSR
PCR primers	Forward cTTccATgAATcAAAgcAgcAc at 243 length 22 bases, Tm 64 Reverse cATccATggcAgAAAcAATAgc at 380 length 22 bases, Tm 63
SSR size	159 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC457
Source Species	Triticum aestivum