GrainGenes Marker Report: Xwmc468-4A
Locus
Xwmc468-4A
Type
Microsatellite
Chromosome
4A
Map
CS/SQ1-4A 98.2 Ta-SSR-2004-4A 37.7
[ Show Nearby Loci ]
Map Data
Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004
Probe
WMC468
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc468 Xwmc0468-4A wmc468 wmc468-4A
Probe for Locus
WMC468
Locus
Xwmc468-4A Xwmc468 wmc468 wmc468-4A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 21g12 Donor: Dupont Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 156 bases located on 4A Trimmed to Ssp1 at 5' end and to insert at 3' end. (CT)21 145 to 186.
Type
PCR SSR
PCR primers
Forward AgcTgggTTAATAAcAgAggAT at 74 length 22 bases, Tm 58
Reverse cAcATAAcTgTccAcTccTTTc at 202 length 22 bases, Tm 58
SSR size
150 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (126-176 bases, complex).
Sequence
WMC468
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc468-4A
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