GrainGenes Marker Report: Xwmc47-5A
Locus
Xwmc47-5A
Type
Microsatellite
Chromosome
5A
Map
Ta-SSR-2004-5A 24
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC47
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc47
[ Show all 6 ]
Probe for Locus
WMC47
Locus
Xwmc47-4B
[ Show all 7 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 6f3 Donor: Dubcovsky, Buenos Aires Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 127 bases located on 4B, 144 bases located on 4B AGR70. Trimmed to insert at 3' end. (CA)8 86 to 101. (3 loci).
Type
PCR SSR
PCR primers
Forward gAAAcAgggTTAAccATgccAA at 30 length 22 bases, Tm 65
Reverse ATggTgcTgccAAcAAcATAcA at 149 length 22 bases, Tm 65
SSR size
141 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence
WMC47
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc47-5A
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