GrainGenes Marker Report: Xwmc470-2D
Locus
Xwmc470-2D
Type
Microsatellite
Chromosome
2D
Map
Ta-SSR-2004-2D 37.1
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC470
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc470 Xwmc470-1A Xwmc470-5A wmc470 wmc470-2D
Probe for Locus
WMC470
Locus
Xwmc470
[ Show all 6 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25d1 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 147 bases located on 3B Trimmed to insert at both ends. Bases 14 to 30 show homology to 5b5 bases 3 to 19 (17 in longest run, maxScore = 35). (CA)7 1 to 14, (CA)9 65 to 82, (CA)6 135 to 146.
Type
PCR SSR
PCR primers
Forward AcTTgcAAcTggggAcTcTc at 39 length 20 bases, Tm 61
Reverse TccccAATTgcATATTgAcc at 165 length 20 bases, Tm 62
SSR size
146 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product.
Sequence
WMC470
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc470-2D
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