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GrainGenes Marker Report: Xwmc473

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Locus	Xwmc473
Type	Microsatellite
Map	Ta-NxW-4D 63.7 Wheat, UC1110 x PI610750 4D 63.5 Wheat, UC1110 x PI610750 4D 63.8
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC473
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc473-4D [ Show all 8 ]
Probe for Locus	WMC473
Locus	Xwmc473-4D [ Show all 9 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25e2 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 111 bases located on 1D, 149 bases located on 4D Trimmed to Ssp1 at 5' end and to insert at 3' end. (CA)27 40 to 93.
Type	PCR SSR
PCR primers	Forward TcTgTTgcgcgAAAcAgAATAg at 6 length 22 bases, Tm 64 Reverse cccATTggAcAAcAcTTTcAcc at 122 length 22 bases, Tm 65
SSR size	138 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product. tested 51C anneal, no product.
Sequence	WMC473
Source Species	Triticum aestivum