GrainGenes Marker Report: Xwmc475-7B
Locus
Xwmc475-7B
Type
Microsatellite
Chromosome
7B
Map
Ta-SSR-2004-7B 58.6
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC475
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc475-5A wmc475 wmc475-5A wmc475-7B
Probe for Locus
WMC475
Locus
Xwmc475-5A Xwmc475-7B wmc475 wmc475-5A wmc475-7B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25f3 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 120 bases located on 5B, 132 bases located on 3A, 140 bases located on 5A, 156 bases located on 7B Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)21 292 to 333.
Type
PCR SSR
PCR primers
Forward AAcAcATTTTcTgTcTTTcgcc at 206 length 22 bases, Tm 62
Reverse TgTAgTTATgcccAAccTTTcc at 338 length 22 bases, Tm 62
SSR size
154 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product (1 sample failed in PCR).
Sequence
WMC475
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc475-7B
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