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GrainGenes Marker Report: Xwmc477-2B

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Locus	Xwmc477-2B
Type	Microsatellite
Chromosome	2B
Map	CS/SQ1-2B 123.8 Louise/Penawawa-2B 49.1 Ta-SSR-2004-2B 63.4
	[ Show Nearby Loci ]
Map Data	Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa
Probe	WMC477
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc0477-2B wmc477-2B wmc477 Xwmc477
Probe for Locus	WMC477
Locus	Xwmc477-2B Xwmc0477-2B wmc477-2B wmc477 Xwmc477
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25b4 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 170 bases located on 2B Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)16 118 to 149.
Type	PCR SSR
PCR primers	Forward cgTcgAAAAccgTAcAcTcTcc at 10 length 22 bases, Tm 65 Reverse gcgAAAcAgAATAgcccTgATg at 155 length 22 bases, Tm 65
SSR size	167 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC477
Source Species	Triticum aestivum