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GrainGenes Marker Report: Xwmc479-7A

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Locus	Xwmc479-7A
Type	Microsatellite
Chromosome	7A
Map	CS/SQ1-7A 35.6 Kofa/UC1113-7A 15 Ta-SSR-2004-7A 22.1
	[ Show Nearby Loci ]
Map Data	Durum wheat, Kofa x UC1113 Durum wheat, Svevo x Ciccio Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004
Probe	WMC479
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc479 [ Show all 9 ]
Probe for Locus	WMC479
Locus	Xwmc479-7A [ Show all 10 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25e4.2 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 199 bases located on 7A Multiple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)16 259 to 290.
Type	PCR SSR
PCR primers	Forward gAccTAAgcccAgTgTcATcAg at 162 length 22 bases, Tm 63 Reverse AgAcTcTTggcTTTggATAcgg at 336 length 22 bases, Tm 63
SSR size	196 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC479
Source Species	Triticum aestivum