GrainGenes Marker Report: Xwmc486-6B
Locus
Xwmc486-6B
Type
Microsatellite
Chromosome
6B
Map
Conan/Reeder-6B 7.14585 Ta-SSR-2004-6B 2.9
[ Show Nearby Loci ]
Map Data
Wheat, Conan x Reeder Wheat, Consensus SSR, 2004
Probe
WMC486
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc486 wmc486 wmc486-6B
Probe for Locus
WMC486
Locus
Xwmc486-6B Xwmc486 wmc486 wmc486-6B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25d7.2 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 213 bases located on 6B Multiple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)28 68 to 123.
Type
PCR SSR
PCR primers
Forward ccggTAgTgggATgcATTTT at 38 length 20 bases, Tm 64
Reverse ATgcATgcTgAATccggTAA at 224 length 20 bases, Tm 63
SSR size
206 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (120-140 bases)
Sequence
WMC486
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc486-6B
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