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GrainGenes Marker Report: Xwmc487-6B

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Locus	Xwmc487-6B
Type	Microsatellite
Chromosome	6B
Map	Ta-SSR-2004-6B 9.2
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Map Data	Wheat, Consensus SSR, 2004
Probe	WMC487
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	wmc487-6B wmc487
Probe for Locus	WMC487
Locus	Xwmc487-6B wmc487-6B wmc487
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25f7 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 163 bases located on 6B Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)20 120 to 159.
Type	PCR SSR
PCR primers	Forward cAAATTTggccAccATTTTAcA at 53 length 22 bases, Tm 63 Reverse cggTTcAATccTTggATTTAcA at 191 length 22 bases, Tm 60
SSR size	160 bases expected, Chinese Spring
Amplification Conditions	tested 51C anneal, no product.
Sequence	WMC487
Source Species	Triticum aestivum