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GrainGenes Marker Report: Xwmc489d

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Locus	Xwmc489d
Type	Microsatellite
Chromosome	3A
Map	Wheat-Composite2004-3A 81
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC489
Possible Orthologs	Xwmc489-1D [ Show all 23 ]
Probe for Locus	WMC489
Locus	Xwmc489a [ Show all 24 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25f8 Donor: Serasem Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 13 to 97 show homology to 7e6 bases 60 to 144 (85 in longest run, maxScore = 75). (CA)13 98 to 123. Bases 98 to 189 show homology to 26f11 bases 81 to 172 (92 in longest run, maxScore = 146). Duplicate with 26f11, 25f8 has priority.
Type	PCR SSR
PCR primers	Forward cgAAggATTTgTgATgTgAgTA at 37 length 22 bases, Tm 59 Reverse ggAcAAcATcATAgAgAAggAA at 247 length 22 bases, Tm 59
SSR size	232 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (various bands 176-270 bases, complex)
Sequence	WMC489
Source Species	Triticum aestivum