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GrainGenes Marker Report: Xwmc48c

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Locus	Xwmc48c
Type	Microsatellite
Chromosome	4A
Map	Wheat-Composite2004-4A 54
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC48
Possible Orthologs	Xwmc48 [ Show all 12 ]
Probe for Locus	WMC48
Locus	Xwmc48-4B [ Show all 13 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6c4 Donor: Dubcovsky, Buenos Aires Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 139 bases located on 4A, 149 bases located on 4D, 190 bases located on 4B AGR75. Trimmed to insert. (GA)9 177 to 194. Not sure if polymorphic. Contains sequence element 104-172 that matches complement of 6a12. Jorge Dubcovsky maps this to all group 4 homoeologues.
Type	PCR SSR
PCR primers	Forward gAgggTTcTgAAATgTTTTgcc at 101 length 22 bases, Tm 64 Reverse AcgTgcTAgggAggTATcTTgc at 202 length 22 bases, Tm 63
SSR size	123 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC48
Source Species	Triticum aestivum