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GrainGenes Marker Report: Xwmc49-1B

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Locus	Xwmc49-1B
Type	Microsatellite
Chromosome	1B
Chromosome Arm	1BS
Map	Ta-SSR-2004-1B 18.6 Ta-Synthetic/Opata-GPW-1B 18.2 Ta-Synthetic/Opata-SSR2-1B 15.8
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Map Data	Wheat, Consensus SSR, 2004 Wheat, Synthetic x Opata, GPW Wheat, Synthetic x Opata, SSR 2
Probe	WMC49
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	wmc49 wmc49-1B
Probe for Locus	WMC49
Locus	Xwmc49-1B wmc49 wmc49-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6d4 Donor: Dubcovsky, Buenos Aires AGR76. Trimmed to insert at 5' end. (CT)19 141 to 178.
Type	PCR SSR
PCR primers	Forward cTcATgAgTATATcAccgcAcA at 82 length 22 bases, Tm 60 Reverse gAcgcgAAAcgAATATTcAAgT at 266 length 22 bases, Tm 62
SSR size	206 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product;
Sequence	WMC49
Source Species	Triticum aestivum