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GrainGenes Marker Report: Xwmc492-3D

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Locus	Xwmc492-3D
Type	Microsatellite
Chromosome	3D
Map	Conan/Reeder-3D 106.894 Louise/Penawawa-3D 0 Ta-SSR-2004-3D 23.5
	[ Show Nearby Loci ]
Map Data	Wheat, Conan x Reeder Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa
Probe	WMC492
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc492 [ Show all 6 ]
Probe for Locus	WMC492
Locus	Xwmc492-3D [ Show all 7 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25e9 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 150 bases located on 3D, 337 bases located on 7B, 7D, 450 bases located on 4D Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 5 to 29 (complement) show homology to 13A1 bases 164 to 188 (25 in longest run, maxScore = 31). (CA)8 260 to 275, (CA)8 278 to 293, (CA)9 296 to 313. Primers share a motif, small inverted repeats near the microsatellite.
Type	PCR SSR
PCR primers	Forward AggATcAgAATAgTgcTAccc at 192 length 21 bases, Tm 57 Reverse ATcccgTgATcAgAATAgTgT at 317 length 21 bases, Tm 58
SSR size	146 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (126-154 bases).
Sequence	WMC492
Source Species	Triticum aestivum